# hwenzym - 2 Data table Absorbance measured at 340nm Run 1 2...

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2) Data table : Absorbance measured at 340nm Run # Abs 15sec Abs 30sec RU (min -1) Velocity (µmole/ min.mL) 1 0.114 0.348 936 4.51 2 0.748 1.039 1164 5.61 3 0.758 0.957 796 3.84 4 0.971 1.232 1044 5.04 5 0.667 0.913 984 4.75 6 0.871 1.145 1096 5.29 7 0.683 0.989 1224 5.90 8 0.851 1.065 856 4.13 9 0.568 0.812 976 4.71 10 0.513 0.710 788 3.80 Average run# 6-10 988 4.76 +/- 0.238 a) RU for run #1 Racker units (RU) = (change in absorbance) (60sec) x 1000 (change in time) (1 min) = (0.348 – 0.114) (60sec) x 1000 (30sec – 15 sec) (1 min) = 936 b) Velocity of run #1 Velocity = (change in absorbance) (60sec) 1 volume of assay 1 1 x 10 6 µmole (change in time) (1 min) volume of NAD Ɛ + path length 1 mole

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Unformatted text preview: = (Abs 30 sec – Abs 15 sec ) (60sec) (cm.mole) (3.0 x 10-3 L) 1 1 x 10 6 µmole (30 sec – 15 sec) (1 min) (6220 L) (0.1 mL) (1.00 cm) 1 mole = (0.348- 0.114) (60sec) (cm.mole) (3.0 x 10-3 L) 1 1 x 10 6 µmole (30 sec – 15 sec) (1 min) (6220 L) (0.1 mL) (1.00 cm) 1 mole = 4.51µmole/min.mL c) RU average calculation RU average = ∑(Ru of runs# 6-10) 5 = (1096 + 1224 + 856 + 976 + 788) 5 = 988 d) Velocity average calculation Velocity average = ∑(Velocity of runs# 6-10) 5 = ( 5.29 + 5.90 + 4.13 + 4.71 + 3.80) 5 = 4.77 µmole/min.mL...
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