Enzymology Laboratory Lab - Nurul Nadzirah Roslan 007544407...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Nurul Nadzirah Roslan 007544407 Enzymology Laboratory Report Introduction: i. Enzymes are catalysts that accelerate the rates of biological reactions. Enzymes are important to biochemistry because enzymes accelerate the reaction rates many orders of magnitude and, by selecting the substances undergoing reaction, determine the specific reaction taking place, these results that we will be able to maintain the living state. This is because other common ways for chemist to accelerate reactions are not available to cells, such as the temperature cannot be raised, acid or base cannot be added, the pressure cannot be elevated or the concentrations cannot be dramatically increased. ii. NAD + + ethanol acetaldehyde + H + + NADH iii. Environmental conditions affecting the enzymes ability to catalyze the reaction that were tested in this lab: a. pH of pyrophosphate buffer b. concentration of substrate which is ethanol c. concentration of ADH enzyme d. temperature of ADH enzyme iv. The standard assay protocol is start by adding 2.2 mL distilled H 2 O into a cuvette, and then 0.5 mL pyrophosphate buffer, followed by 0.1 mL ethanol and lastly is 0.1 mL of ADH. The mixture solutions is then covered with parafilm and mixed by inverting the cuvette 2-3 times. The cuvette then is placed into the spectrometer and the lid is closed. The wavelength of the spectrometer is made sure set at 340 nm and then the spectrometer is set blank by pressing the “0 Abs” button. After the absorbance reading is displayed 0, the cuvette is took out immediately and then 0.1 mL NAD + is added, the timer is begin simultaneously as the adding. The solution is again mixed by inverting the cuvette 2-3 times before placing inside the spectrometer. The absorbance then is read at every 15 seconds up to 75 seconds. 1
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
v. The wavelength 340 nm is chosen because the maximum wavelength for the reduced form of NAD + which is NADH, is 340 nm.
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern