587 08 wk09c tem_sem std

587 08 wk09c tem_sem std - Electron Microscopy Modified by...

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1 Electron Microscopy Modified by Dr. H. C. Allen 05/22/08 Hendrik O. Colijn OSU Campus Electron Optics Facility Why EM? • Rayleigh Criterion for electrons (small β ) β λ δ 61 . 0 = λ≈ 0.003 nm for electrons compared to 500 nm for light – over 100,000 x smaller!
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2 Electron vs. Light Microscopy • Electrons vs. photons • Probe propagation through sample • Strong interactions with matter means a thin sample and possible radiation damage • Also requires vacuum • Magnetic lenses -- charged particles/won’t go through glass. Sample constraints • Vacuum compatible • Electron-sample interaction different than for light. Biological samples (and polymers) often need stained. Need different stains than for LM. • Able to withstand radiation dose, needs to be somewhat conductive
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3 Ni Superalloy Yew Leaf
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4 Mushroom Spores Chili Root Hair
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5 Teflon Tape NaCl crystals
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TEM Imaging You are looking at a 2D projection of a 3D object. TEM images
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587 08 wk09c tem_sem std - Electron Microscopy Modified by...

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