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sayres_notes - The Dark Reactions 6CO2 12H2O 18ATP 12NADPH...

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The Light Reactions The Light Reactions The Dark Reactions The Dark Reactions 6CO 2 + 12H 2 O + 18ATP + 12NADPH 6C(H 2 O) + 6O 2 + 18 ADP + 18Pi + 12 NADP + 6H 2 O
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Two Types of Reaction Centers Two Types of Reaction Centers
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Rhodobacter viridis Rhodobacter viridis Bacterial Photosynthetic Reaction Center Bacterial Photosynthetic Reaction Center Non-oxygenic Photosynthesis Non-oxygenic Photosynthesis First membrane protein structure resolved at atomic levels of resolution by X-ray diffraction. Michel, Deisenhoffer and Huber - Nobel Prize in Chemistry, 1989 Isolation and Crystallization: Membranes were solubilized with octyl glucopyranoside – detergent mimics natural membrane lipids Crystalized by vapor diffusion against 2.2 M ammonium sulfate for 2-3 weeks Unit dimensions of BRC: 130 x 70 x 30 Å
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Bacterial Photosynthetic Reaction Bacterial Photosynthetic Reaction Center, Quinone Type-II Center, Quinone Type-II L subunit L subunit- Binds chlorophyll, pheophytin and quinone cofactors involved in electron transfer M subunit M subunit – Binds chlorophyll, pheophytin and quinone cofactors involved in electron transfer H subunit H subunit – Stabilizes complex Cyt C Cyt C – extrinsic protein binds four hemes involved in electron transfer Cofactors Cofactors : 4 hemes, Chlsp, 2 Chlm, 2 Pheophytin, 2 quinones, 1 non-heme Fe
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BRC Subunit Composition Peptide Amino Acids Transmembrane Spans Cofactors L 273 5 4Bchl, 2Pheo, 1Fe, Qb M 323 5 4Bchl, 2Pheo, 1Fe, Qa H 258 1 None Cyt C 336 0 4 hemes Transmembrane spans are 21-28 residues in length and alpha helical. L and M subunits have 25% sequence identity. 216 alpha carbon (backbone) atoms of the L and M subunits superimpose within 1.2 Å. Two-fold (C2) axis of symmetry.
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Cofactor Orientation Cofactor Orientation Chlsp +0.45 eV Qa -0.2 eV eV = 0.7
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Protein Cofactor Interactions Protein Cofactor Interactions Cofactor Ligands Chlsp L-H173, M-H200 Chlm L-H153, M-H180 Menaquinone Qa
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