640_chp8 - protein variation how do we measure variability...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
1 protein sequences protein variation • how do we measure variability – structural similarity? how? • immunology • enzyme activity • indirect structural differences (electrophoresis) amino acid similarity (sequence) amino acid sequencing • method developed in 1950’s by Sanger – Insulin sequence - 1953 A chain = 21 a.a. C chain = 30 a.a. B chain = 33 a.a. SS S S S S protein comparisons •by 1963 significant comparisons made and conclusions drawn: – The same protein from different organisms is similar in amino acid sequence (but usually not identity) α -hemoglobin amino acid sequences 70/143 identical protein comparisons • by 1963 significant comparisons made and conclusions drawn: – The same protein from different organisms is similar in amino acid sequence (but usually not identity) – The more similar two organisms are thought to be, the more similar their protein sequences are • molecular clock (Zuckerkandl and Pauling, 1963)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 AMINO ACID DIFFERENCES AND TIMES OF DIVERGENCE (CYTOCHROME C)
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 07/17/2008 for the course EEOB 640 taught by Professor Fuerst during the Spring '05 term at Ohio State.

Page1 / 3

640_chp8 - protein variation how do we measure variability...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online