640_chp9 - DNA VARIATION SEQUENCES: INDIRECT AND DIRECT...

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Unformatted text preview: DNA VARIATION SEQUENCES: INDIRECT AND DIRECT nucleic acid variation indirect DNA/DNA hybridization enzymatic degradation restriction enzyme digestion (RFLP) amplification polymorphism (RAPD) repeat sequence variation (VNTR / STR) direct sequencing nucleic acid variation indirect DNA/DNA hybridization enzymatic degradation restriction enzyme digestion (RFLP) amplification polymorphism (RAPD) repeat sequence variation (VNTR / STR) direct sequencing DNA / DNA HYBRIDIZATION DNA / DNA HYBRIDIZATION MELTING PROFILE FOR T4 PHAGE DNA / DNA hybridization Distance = based on T m50 50% of hybrid molecules remain as heteroduplexes relationship between T m and % sequence divergence 1 o C T m = ~ 0.7 - 1.7% sequence divergence DNA / DNA hybridization used for species comparisons not used for population comparisons within species variation equivalent to error rate of method Example: bacterial taxonomy 30% sequence divergence = species OSTRICH OSTRICH / RHEA HETERODUPLEX human chimp bonobo gorilla orangutan gibbon T m chimpanzee 1.8 bonobo 1.9 0.7 gorilla 2.4 2.1 2.3 orangutan 3.6 3.7 3.7 3.8 gibbon 5.2 5.1 5.6 5.4 5.1 baboon 7.7 7.7 8.0 7.5 7.6 7.4 DNA / DNA hybridization and primate relationships Sibley & Ahlquist, 1984 nucleic acid variation indirect DNA/DNA hybridization...
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640_chp9 - DNA VARIATION SEQUENCES: INDIRECT AND DIRECT...

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