640_chp10 - nucleic acid variation indirect DNA/DNA...

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nucleic acid variation indirect – DNA/DNA hybridization enzymatic degradation restriction enzyme digestion (RFLP) amplification polymorphism (RAPD) – repeat sequence variation (VNTR / STR) direct – sequencing DNA POLYMORPHISMS AMPLIFICATION INDIRECT ESTIMATES OF SEQUENCE DIVERGENCE – PCR BASED MEASURES RAPD VARIATION IN PRIMER SITES STS SEQUENCE TAGGED SITES with RFLP RAPD randomly amplified polymorphic DNA PCR based technique – use of single short oligonucleotide primer - often 10 base PRIMER 1 PRIMER 1 RAPD randomly amplified polymorphic DNA PCR based technique – use of single short oligonucleotide primer - often 10 base primers located as inverted repeats PRIMER 1 RAPD randomly amplified polymorphic DNA Multiple “loci” amplify – many different locations of 10 bp primer site • Any location with inverted repeat can amplify PRIMER 1
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randomly amplified polymorphic DNA Multiple “loci” amplify – nucleotide changes in one or both sites can “destroy” a band and produce a polymorphism no amplification ! PRIMER 1 RAPD randomly amplified polymorphic DNA “+” alleles show dominance + / + RAPD randomly amplified polymorphic DNA “+” alleles show dominance “-” allele is recessive X + / + + / - RAPD randomly amplified polymorphic DNA “+” alleles show dominance “-” allele
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This note was uploaded on 07/17/2008 for the course EEOB 640 taught by Professor Fuerst during the Spring '05 term at Ohio State.

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640_chp10 - nucleic acid variation indirect DNA/DNA...

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