g640P105 - MolGen 640 Problem #1. - Spring 2005 problem due...

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MolGen 640 Problem #1. - Spring 2005 problem due Wednesday April 13 A sample of the butterfly Carmeniae ohioii is collected in Columbus, Ohio, and studied for genetic variation at several loci. The loci included a morphological locus for wing coloration, and a biochemical locus, the enzyme locus Phosphoglucomutase-1 (PGM-1), using protein electrophoresis. All the females that were collected as part of this study have been inseminated in the wild. Previous work by members of the Department of Evolution, Ecology and Organismal Biology has indicated that females mate only once before laying eggs. The species shows extreme limitation of habitat, using only groves of Buckeye trees for food. Females do not move more than 100 yards from where they pupate, so that female "populations" are very discrete. Males, however, will migrate up to 10 miles a day, drawn to females by the presence of mating pheromones. The sample of females for this study was collected from the Buckeye grove on the OSU campus. After collection, females were allowed to lay eggs in the laboratory, were scored for the type of wing coloration polymorphism that they carried, and then were ground up for biochemical studies. (This approach is often called the "find 'em and grind 'em" method of population biology). From the batch of offspring obtained from each single female, a single randomly selected larval offspring was chosen for biochemical analysis. The larval offspring could not, of course, be analyzed for wing coloration. Color polymorphism: Two phenotypes are observed in this population of Carmeniae ohioii , red (actually scarlet) and grey. Differences are caused by alternative alleles at one locus. It has also been found that the locus is sex-linked in Carmeniae ohioii . Previous studies have shown that the grey allele is completely dominant to the red allele in females. The table shows the frequency in the sample of mothers with particular wing color phenotypes.
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This note was uploaded on 07/17/2008 for the course EEOB 640 taught by Professor Fuerst during the Spring '05 term at Ohio State.

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g640P105 - MolGen 640 Problem #1. - Spring 2005 problem due...

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