Column Overview and Analysis - CHM 343 Separations Methods...

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CHM 343 Separations Methods Column Details and Analysis Name: Choon Liang Tan Bronco ID: 007156760 Class: CHM343 L Professor: Dr. Hossein Ahmadzadeh Date: 03.04.08 1
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Varian Analytical Instruments: Microsorb – MV Column This instrument was used in the reversed phase HPLC machine. This column belongs to a product group of C18 columns, meaning a family of columns containing a backbone of 18 carbon atoms attached onto the silicone, and acting as the stationary phase resin. Its brand and model name is Microsorb. Under this brand, there are a total of 11 selections, ranging from 20 x 20mm to 250 x 4.6mm. This selection also has ID ranges from 2 to 4.6mm and column lengths available from as short as 2cm up to 25cm. The particle sizes allowed are between 3 to 8 μm and pore sizes from 60 to 300 angstroms. It is suitable for the study of compounds such as the ascorbic acid, benzoic acid and caffeine. Analyzing those types of analytes, this column will be able to give out a good resolution for their detection, however one must only know how to configure the exact methanol/water ratio correctly to get the best resolution possible. Restek Corp: MXT-5 Column The column used for the Kovats retention indices, the non-polar column was the MXT-5. This product made by Restek Corp also is provided in many shapes and sizes.
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