Dristan Nasal Spray - Simultaneous UV Spectrophotometric...

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Simultaneous UV Spectrophotometric Analysis of The Active Ingredients of Dristan Nasal Spray Author: Tan Choon Liang Bronco ID: 007156760 Lab Partner: Diaz Yolanda Date: 10.22.07
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Abstract: Samples of nasal sprays and an unknown were run through UV Spectrophotometry analysis to determine the exact concentrations of two active ingredients Pheniramine Maleate and Phenylephrine Hydrochloride. The samples were put through double-beam instruments and standard solutions were analyzed before the unknowns and nasal sprays. This allowed a calibration to be made and the final concentrations of them both inside the unknowns and nasal sprays to be calculated. It was concluded that the unknown contained exactly 12.0482ppm of Pheniramine Maleate and 25.7070ppm of Phenylephrine Hydrochloride. The nasal spray (100%) contained 3690.0369ppm of Pheniramine Maleate and 188.1468 ppm of Phenylephrine Hydrochloride. This discovery was made by diluting concentrated samples of standard solutions in 10 and 20ppm. With the use of certain equations the concentration of both ingredients in the two samples were found. The absorptions were caused by electronic transitioning in the spectrophotometer. Introduction: The analysis used was UV Spectrophotometry and it was done with a Double- Beam instrument. This meant that the machine didn’t require you to put in the reference sample (blank), have to take it out and replace it with the actual sample. They are run through quartz cuvettes because ultra-violet radiation is absorbed in glass cuvettes. Instead you put in both blanks and samples in the machine simultaneously and it will run the samples one directly after the other with the help of a motorized sector mirror. This provides better compensation for instrumental fluctuations. The UV Spectrophotometry analysis method allows quantitative analysis of highly conjugated organic compounds. It allows us to determine the exact concentration (ppm) of a solution. In this experiment we are trying to find out the concentrations of two specific ingredients in Nasal Sprays, Pheniramine Maleate and Phenylephrine Hydrochloride. This method follows the theories of Beer’s Law, which states that under certain conditions, the absorptions of a solution are directly proportional to the solution’s concentration. Pheniramine Maleate is one of the two ingredients. It is anti-histamine that is used to treat allergic conditions like the hay fever or uric aria. The main brand names that sell this ingredient in their nasal sprays are Avil by Aventis. This is generally sold in
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