CHM 329L Experiment 2 - CHM329L Experiment 2 Plasmid DNA...

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CHM329L Experiment 2: Plasmid DNA 11.01.07 CHM 329L Experiment 2: Plasmid DNA Group Lab Report Authors: Nur Raudhah Rahman (007156747) Tan Choon Liang (007156760) Date: 11.01.07 1
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CHM329L Experiment 2: Plasmid DNA 11.01.07 1. Figure 1.1: Image of Gel and 4 bands, uncut & cut Plasmids 1 and 2 2. Table 1.1: Raw Data and Calculated Values of Rm and log (size of DNA in bp) DNA size: (bp) Log(DNA size): (bp) Distance Traveled by DNA: (cm) Rm: (cm) 702 2.8463 5 1 1264 3.1017 4.1 0.82 1371 3.138 3.9 0.78 1929 3.2853 3.35 0.67 2323 3.366 3.15 0.63 3675 3.5653 2.6 0.52 The standard plot had y = log(DNA size in bp), versus x = Rm. All distances were derived from Figure 1.1 and the exact cm are found on the right hand side of that figure. 2
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CHM329L Experiment 2: Plasmid DNA 11.01.07 Calculations: Rm = Distance Traveled by DNA Distance Traveled by Dye Distance Traveled by our Dye was 5.0cm. This remains as a constant value for all DNA sizes. Example of Calculations: DNA size: (bp) Log(DNA size): (bp) Distance Traveled by DNA: (cm) Rm: (cm) 1371 3.138 3.9 0.78 Rm = 3.9cm = 0.78 5.0cm 3. Calculating the size of the linear DNA Fragment for cut EcoRI Plasmid # 1 EcoRI Plasmid: Rm: Size of Linear DNA Fragment: (bp) Cut # 1: 0.38 5493.3086 Example of Calculation of Size of Linear DNA Fragment: EcoRI Cut # 1: Rm = 0.38 Size of Linear DNA Fragment = anti-log (y value from Figure 1.2) Equation of the Line: y = -1.4657x + 4.2968 (derived from Figure 1.2) y = -1.4657 (0.38) + 4.2968 y = 3.739834 Size of Linear DNA Fragment = anti-log (3.739834) = 5493.3086 4. Calculating the size of the Linear DNA Fragment for cut EcoRI Plasmid # 2 EcoRI Plasmid: Rm: Size of Linear DNA Fragment: (bp) Cut # 2: 0.60 2614.4480 3
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