Amphibian water retention lab

Amphibian water retention lab - Josh Young Endocrinology...

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Josh Young 4/03/08 Endocrinology Lab Linda Malmgren Lab Report: Amphibian Water Retention Introduction: Even in small amounts, ADH in human’s results in antidiuresis, as water excretion is decreased by the kidneys. ADH is associated mainly with water balance. In the nephron it is the primary hormone in regulating water balance. Thus it also is able to promote water reabsorption by the nephron as it increases water permeability of epithelial cells. Vasopressin increases ADH which in turn turns on rennin. ADH is secreted in the posterior pituitary and is a nine amino acid peptide. ADH mainly acts on the kidney, where it conserves water loss by reducing the amount of water in urine. ADH works by binding to cell surface receptors in the kidney, promoting reabsorption of water back into circulation. Reabsorption happens as it stimulates special water channel membranes in the kidney tubules. These water channels act to transport water back into the blood, which leads to a decrease in plasma osmolarity. As ADH is an antidiuretic, it would be expected that over time, each amphibious specimen’s weight would increase. As the amphibians studied and the ones we used in class all had varying habitats and thus varying amounts of water present, one could assume their responsiveness to ADH would also vary. From the species studied, here is a table representing known data:
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Table 1 Materials and methods: See attached lab handout. Results: Listed below is the tabled data of the five different species of amphibians that were experimented on. In each species there was at least one control and one experimental specimen. Weight was recorded initially (t = 0) and at every 30 minute period after for 120 minutes. Weight was measured in grams.
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