Lecture 2 10

Lecture 2 10 - Lecture 2 10/01/07 Cell Biology Techniques...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 2 10/01/07 Cell Biology Techniques Model Organisms- how we study Cell Biology depends on how easily they are studied Genetically tractable (can we make crosses between them, are they diploid/haploid genomes?) How rapid their development is Their size (smaller in size usually than species they are representing) How readily available they are If they are amenable to asking certain questions o Are they helpful or easy to see? o E.g. Zebrafish are clear, they have see through embryos, which is helpful to study reproduction Resources available- if there are sequence genome and mutant stock available, stock center, order knockouts or cDNAs Mammalian Tissue Cultures: 2 types 1.) Primary Culture- derived directly from a tissue -obtain tissue from organism, digest with enzymes, feed it in tissue culture dish, allow cells to grow in culture Advantages- Act as differentiated cells, will continue to act like tissue they came from -e.g. fibroglast cells continue to make collagen as though they are still in body Disadvantages- Will eventually die because cells will stop dividing 2.) Immortalized cell line- derived from tumor or cancer cells or derived from tissue, but treated with tumor inducing viruses/chemicals -immortal, never stop growing unless you stop feeding it -can freeze them, resource can last for long time - Hela cells- around since 1951, most commonly used tissue cells
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 07/21/2008 for the course BICD 110 taught by Professor Mel during the Fall '06 term at UCSD.

Page1 / 3

Lecture 2 10 - Lecture 2 10/01/07 Cell Biology Techniques...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online