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Unformatted text preview: -Proteins in secretory pathway are translated at ER-Signal sequence is both necessary and sufficient Necessary because if it was removed, protein will longer be part of secretory pathway Sufficient because if you add this to a non-secretory protein (i.e. cytoplasm protein) it will become part of the secretory pathway Signal sequence is used for import of protein into ER-Signal Recognition Particle (SRP) Recognizes signal sequence Bind to ribosome and signal sequence Signal sequence is short chain of hydrophobic amino acids (8-15) SRP has hydrophobic pocket for signal sequence- translation gets paused temporarily SRP sees new protein being made, recognizes signal sequence, pauses translation because protein is supposed to be part of ER, so stop to prevent misfolding When the SRP/ribosome complex binds to the SRP receptor, dissociation happens by the hydrolysis of GTP GDP Conformational change causes the SRP to dissociate from the receptor and ribosome...
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