Lecture 3 Tumor Viruses

Lecture 3 Tumor Viruses - Lecture #3 Tuesday 4/8/08 -1-...

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Lecture #3 – Tuesday 4/8/08 - 1 - Announcements: - Grading . Students will be assigned letter grades (A-F with +/-) based on a midterm and a final. Grading will be curved, but the curve is dependent on the class’ performance. In other words, there is no pre-set curved grading scale. - Book. Use a book as a reference for the lecture material. You are not required to know every detail in the book, just the stuff relevant to the lecture topics. o But keep in mind, the book was written by one of the foremost authorities in Cancer Biology. It’s not a hard read and there are a lot of pretty pictures. It’s all good stuff. - Sections. There are no sections for this class, but feel free to email and ask questions. If you have questions regarding the lecture notes, email me [email protected] , if you have questions about the course material, email Dr. Johnson, [email protected] . In the last lecture, we went over some of the history of cancer research, next we’ll focus on the molecular and cellular aspects of the disease. Disease that occurs inside an organism/mammal. Mice and human beings. Mice tend to be the experimental model. People equate transformation in a tissue culture dish to what is happening in a human. Very different from each other actually. Cancer is studied at the molecular and cellular level but it exists in a context more complex than in a dish. You must take into account the immune and vascular systems, organs, etc. This is not always obvious, many people equate in vitro with in vivo . But the two are very different. As you learn more about cancer research, always question the clinical relevance of the experiments you learn about. In this class, we will be discussing mouse models in relation to human cancers because it is the most frequently used model system. Mass of cells that need a blood vessel, interact with immune system, obstruct organ. Cancer Cell Characteristics There is a distinction between tissues initially derived from and organ/patients and tissues meant for tissue culture (TC). Primary cells/cell culture : cells that come directly from the animal tissue/source. Won’t grow very long. Become senescent. - Even if these cells are supplemented with growth factors (GF) and media (which includes amino acids, sugars, isotonic buffers, etc.), they won’t grow very long in a dish. - Instead, the cells become senescent (stop growing). They cells may be able to live in the dish for a long time (so long as you keep supplementing it with new media), but they cease to grow . - If we succeed to adapt the primary culture into TC dish in such a way that we continue to grow it and passage it over and over again, we immortalize the cells, and they no long become senescent. You can pass immortalized cells and they will continue to live and divide (grow).
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Lecture #3 – Tuesday 4/8/08 - 2 - “Pass” : Enzymatically dissociating the cells from the plastic dish on which they grow and re-plate them onto a new dish with new media. -
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This note was uploaded on 07/21/2008 for the course BIMM 134 taught by Professor Johnson during the Spring '08 term at UCSD.

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Lecture 3 Tumor Viruses - Lecture #3 Tuesday 4/8/08 -1-...

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