Experiment 3: Separation and Purification of the Components of an Analgesic Tablet Lauryn Reinhardt Albany College of Pharmacy and Health Sciences: Organic Chemistry: Lab Section K September 29 2015 In this experiment, the three active ingredients of Excedrin, aspirin (acetylsalicylic acid/ASA), acetaminophen (ACE), and caffeine (CAF), were extracted and the aspirin was purified. Excedrin tablets were crushed and mixed with ethyl acetate (EA). The supernatant containing the aspirin, acetaminophen, and caffeine was spotted onto a pre-prepared TLC plate and marked as spot one. The supernatant was poured into a separatory funnel and the solid binders were discarded. An extraction of the basic aqueous layer “NaHCO 3 layer – ASA” was performed using 5% wt. NaHCO 3 . The mixture remaining was spotted at position two. An extraction was then done using 4M HCl to separate the acidic aqueous layer “HCl layer – CAF”.
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- Spring '09
- TLC plate