Post Lab Thin Layer Chromatography

Post Lab Thin Layer Chromatography - Thin Layer...

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Thin Layer Chromatography Christina Wolverton 11 October 2016 TA: Alex Reese Results: R f = distance traveled by compound / distance travelled by solvent Plate 1: Ibuprofen: 2.91cm/3.80cm = 0.766 Caffeine: 0.94cm/3.80cm = 0.247 Acetaminophen: 2.20cm/3.80cm = 0.579 Aspirin: 3.40cm/3.80cm = 0.895 Plate 2: #1- 546123E: 3.47cm/3.65cm = 0.951 #2- 635142F: 3.5cm/3.65cm = 0.959 2.25cm/3.65cm = 0.616 #3- 324561E: 2.00cm/3.65cm = 0.545 0.89cm/3.65cm = 0.244 Plate 3: #4- 612345A: 3.17cm/4.51cm = 0.703 1.30cm/4.51cm = 0.288 #5- 152346D: 1.30cm/4.51cm = 0.258 #6-561234A: 2.88cm/4.51cm = 0.639
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Discussion/Conclusion: From the TLC plates, R f values were calculated. From the R f values, each dot in each lane could be matched to a control. The R f values for the unknown compounds were occasionally significantly different from the R f values of the control compounds. This is most likely due to poor blotting technique in which too much of the compound was blotted on to the TLC paper. In these cases we used the R f values in conjunction with what appeared to look similar to the controls because the solvent fronts were all a similar distance from the origin.
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  • Fall '07
  • Hubbard
  • rf values, layer chromatography

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