Chem 476 HW 3 Due Sept 12, 2007 “Imaging of Proteins in Cells” Name:________________________ Please complete all questions. Do your work on additional sheets of paper and stapleto the back of this sheet. Do not put your answers on this page. You may work with your classmates on this and all homework assignments. I will grade the same threequestions for all students and post the answers to the questions after they are due. I.) Problems from the textbook and supplement (do not hand in). Answers are in the textbook (short answers) and supplement (full answers to text AND supplement), but work through on your own first. : Text:3.1, 3, 5-7, 10-15, 17, 19 Supp:3.1-3, 5, 7-8, 10-14 II.) Additional Problems to hand in:Paper:“Squint Busters” (2006) C&EN 84, 49-52. Define these terms:(Feel free to use other resources but provide your sources and any url used.) •NIH, NICHD, and 1 other NIH institute (see http://www.nih.gov/icd/) •PALM •STORM •STED •quelling General Questions1.) What is the Abbe, or diffraction, limit? 2.) How was STED used to overcome the Abbe ‘limit’? Make a sketch to help explain.
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NIH, conventional fluorescent microscope, bp dsDNA molecule, Abbe `limit'?