bmb428_problemset15 - BMB428 Physical Chemistry with...

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BMB428 Dr. Song Tan Physical Chemistry with Biological Applications Fall 2007 1 Problem Set 15: Ligand Binding Due Oct 23, 2007 1. The binding of a substrate to an enzyme is found to increase if the ionic strength of the solution increases. Provide possible molecular explanations for this effect. 2. Problem 6.28 (p. 232): An equilibrium dialysis experiment showed that the concentration of free ligand, bound ligand and free protein are 1.2 x 10 -5 M, 5.4 x 10 -6 M, and 4.9 x 10 -6 M, respectively. Calculate the dissociation constant for the reaction PL P + L. Assume there is only one binding site per protein molecule. 3. Equilibrium dialysis experiments are the most common experimental technique to measure ligand binding to a macromolecule. In a typical experiment, compartment A contains enzyme. This chamber is separated from compartment B, which contains a known concentration of ligand X. This allows samples to be withdrawn from compartment B at various time points for analysis. Suppose that the
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This note was uploaded on 07/23/2008 for the course BMB 428 taught by Professor Tan during the Fall '07 term at Pennsylvania State University, University Park.

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