bmb428_problemset26 - BMB428 Physical Chemistry with...

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BMB428 Dr. Song Tan Physical Chemistry with Biological Applications Fall 2007 1 Problem Set 26: Enzyme Inhibition Due Dec 11, 2007 1. Consider the following modification of the Michaelis Menten mechanism: E + S ES k 1 k -1 E + I EI k 3 k -3 k 2 E + P In this mechanism, a second molecule, I, can bind to the free enzyme and prevent (or compete with) the binding of substrate. (a) If the [S] is much greater than [I], would you expect the velocity (d[P]/dt) for this mechanism to be different than for the Michaelis Menten mechanism? (b) Draw a Lineweaver Burk plot for this mechanism. The first line should have [I]=0, and the second should have [I] present at a reasonable concentration. Remember your answer to part (a) to decide if you are going to have a slope effect or an intercept effect or both. 2. In this question, you will derive d[P]/dt for the competitive inhibition mechanism in question 1. (a)
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This note was uploaded on 07/23/2008 for the course BMB 428 taught by Professor Tan during the Fall '07 term at Pennsylvania State University, University Park.

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bmb428_problemset26 - BMB428 Physical Chemistry with...

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