Q3a - Chem 476 September 14, 2007 Quiz 3 Name:_Answer Key_...

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Chem 476 September 14, 2007 Quiz 3 Name:__ Answer Key _ Please complete all questions. You have 10 minutes for the quiz. Matching Questions Use the following to answer questions 1-8: Choose the correct answer from the list below. Not all of the answers will be used. WRITE OUT YOUR ANSWER, DON’T USE JUST THE LETTER. a) HPLC b) specific activity c) MALDI-TOF mass spectrum d) zonal centrifugation e) nascent f) SDS g) epitope h) Svedberg i) centrifugation j) overlap peptides k) affinity chromatography 1. The ratio of enzyme activity relative to total protein is called __ b) specific activity __. 2. The first step in protein purification from a homogenate is usually _ i) centrifugation _. 3. __ k) affinity chromatography _ A type of purification that is based on the attraction of the protein for a particular chemical group. 4. _____ f) SDS _ can be added prior to gel electrophoresis to denature the proteins.
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This note was uploaded on 07/23/2008 for the course CHEM 476 taught by Professor Bevilacqua,philip during the Fall '07 term at Penn State.

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Q3a - Chem 476 September 14, 2007 Quiz 3 Name:_Answer Key_...

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