Chemistry 476 (Biological Chemistry)
Due: Monday December 3, 2007
This is a take-home exam, which counts as 20/130
of your final grade. It is based largely on
reading and understanding a paper from the literature on enzymology and teaching ourselves
some nucleotide biosynthesis (Chpt 25 from BTS). You are welcome to use any books or
Internet resources, but should reference them as appropriate in your answers. You cannot
consult within another person, including your classmates or other professors or students. There
are 12 questions worth a total of 172 points. Please attach pages with your answers and initial
the top of each page.
I understand and attest that I have followed it.
(The two papers are posted on the class website.)
Purine Nucleoside Phosphorylase: Catalysis, Structure, and
Inhibition” Taylor, E. A., Rinaldo-Matthis, A., Ghanem, M., Hazelton, K., Cassera, M. B., Almo,
S. C., and Schramm, V. L. (2007)
“Enzymatic Transition State Theory and Transition State Analogue Design”
Schramm, V. L. (2007)
J. Biol. Chem. 282
: Berg, Tymoczko, and Stryer. “Biochemistry” 6
ed. Chpt 25.
1.) (12) Define these terms:
Feel free to use other resources and to work together, but give your
source and any url used. Do NOT just copy the answer from somewhere and put quotes around
it. Do say it in your own words then give the reference.
is an organism that is unable to synthesize certain organic compounds
compare nucleoside to a nucleotide
A nucleoside is the base plus the sugar. A nucleotide
is a base, sugar, and phosphate
mosquito vector (note this is a different usage of the word ‘vector’ than used earlier in the
semester for cloning)
A mosquito that itself does not cause malaria but spreads the
disease by carrying a parasite that causes malaria.
(what is it and why study it)
A group of mosquitoes that carries
protozoan parasites (
) that cause malaria
Protozoan parasites that cause malaria.
Drug resistance (what is it and what causes it)
This is the ability of a pathogen to be
unaffected by a previously effective drug. It is caused by evolution in which changes to
the genome lead to altered proteins and RNA that no longer interact with the drug in the