Exam3a - Chemistry 476(Biological Chemistry Examination 3...

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1 Chemistry 476 (Biological Chemistry) Examination 3 Due: Monday December 3, 2007 Name: ___________________ ____________________ (First) (Last) This is a take-home exam, which counts as 20/130 th of your final grade. It is based largely on reading and understanding a paper from the literature on enzymology and teaching ourselves some nucleotide biosynthesis (Chpt 25 from BTS). You are welcome to use any books or Internet resources, but should reference them as appropriate in your answers. You cannot consult within another person, including your classmates or other professors or students. There are 12 questions worth a total of 172 points. Please attach pages with your answers and initial the top of each page. I understand and attest that I have followed it. Signature:_______________________________ Resources: (The two papers are posted on the class website.) Paper: Anopheles gambiae Purine Nucleoside Phosphorylase: Catalysis, Structure, and Inhibition” Taylor, E. A., Rinaldo-Matthis, A., Ghanem, M., Hazelton, K., Cassera, M. B., Almo, S. C., and Schramm, V. L. (2007) Biochemistry 46 , 12405-12415. Background Paper: “Enzymatic Transition State Theory and Transition State Analogue Design” Schramm, V. L. (2007) J. Biol. Chem. 282 , 28297-28300. Textbook : Berg, Tymoczko, and Stryer. “Biochemistry” 6 th ed. Chpt 25. 1.) (12) Define these terms: Feel free to use other resources and to work together, but give your source and any url used. Do NOT just copy the answer from somewhere and put quotes around it. Do say it in your own words then give the reference. auxotroph is an organism that is unable to synthesize certain organic compounds compare nucleoside to a nucleotide A nucleoside is the base plus the sugar. A nucleotide is a base, sugar, and phosphate mosquito vector (note this is a different usage of the word ‘vector’ than used earlier in the semester for cloning) A mosquito that itself does not cause malaria but spreads the disease by carrying a parasite that causes malaria. Anopheles gambiae (what is it and why study it) A group of mosquitoes that carries protozoan parasites ( Plasmodium falciparum ) that cause malaria Plasmodium falciparum Protozoan parasites that cause malaria. Drug resistance (what is it and what causes it) This is the ability of a pathogen to be unaffected by a previously effective drug. It is caused by evolution in which changes to the genome lead to altered proteins and RNA that no longer interact with the drug in the same fashion.
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2 2.) (10) What is the central biological hypothesis of this paper? In other words, what are the authors hoping to find that will allow them to cure a disease. Answer in general terms, not specific molecular terms. HsPNP and AgPNP will operate by different mechanisms, allowing
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Exam3a - Chemistry 476(Biological Chemistry Examination 3...

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