Q5a - solid liquid gas phase(Circle your answer 5(5 PCR has...

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Chem 476 October 5, 2007 Quiz 5 Name:________________________ Please complete all questions. You have 10 minutes for the quiz. 1.) (20) Restriction enzymes can be divided into two classes on the basis of the DNA ends they leave. Provide the classes below and give an example of each. a.) Class I:______ Sticky ends _________ example: Draw either a 5’ or 3’ overhang a.) Class II:________ Blunt ends _________ example: Draw = + = 2.) (5) Southern blotting is a way to detect the presence of a specific DNA sequence . 3.) (30) Sanger sequencing relies on a specific type of modified DNA sugar. Name it and sketch the sugar below (for the base, just use ‘N’). Name: dideoxy nucleotide Sketch: see p138 of text 4.) (5) DNA synthesis occurs in the
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Unformatted text preview: solid, liquid, gas phase. (Circle your answer) 5.) (5) PCR has revolutionized molecular biology, forensics, and the legal system. What does PCR stand for? polymerase chain reaction 6.) (5) Ampicillin resistance allows bacteria to grow in the presence of ampicillin. This is conferred on a circular piece of DNA known as a vector or plasmid . 7.) (30) While digging a foundation for a barn, an Amish farmer finds a large bone and donates it to Penn State. How might you figure out what organism the bone came from? List at least 5 different steps in your analysis. A.) PCR B.) ligation C.) restriction digest of PCR product and vector D.) ligate digested pieces E.) Clone into E. coli F.) Sequence G.) BLAST answer...
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