Problem_set_5 - Problem Set#5 BMB 401 Spring 2004 Problems from Lehninger 1 2 3 4 5 6 7 8 9 10 12 Problem 1 The kcat for alkaline

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
BMB 401 Spring 2004 Problems from Lehninger: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, Problem 1 – The k cat for alkaline phosphatase-catalyzed hydrolysis of methylphosphate is approximately 14 sec -1 at pH 8 and 25°C. The rate constant for the uncatalyzed hydrolysis of methylphosphate under the same conditions is approximately 1 x 10 -15 sec. What is the difference in the free energies of activation of these two reactions? Problem 2 – For a Michaelis–Menten reaction (shown below), k 1 = 7 x 10 7 M -1 sec -1 , k -1 = 1 x 10 3 sec -1 , and k 2 = 2 x 10 4 sec -1 . What are the values of K s and K M ? Does substrate binding approach equilibrium or does it behave more like a steady-state system? Problem 3 – The following kinetic data were obtained for an enzyme in the absence of any inhibitor ( 1 ), and in the presence of two different inhibitors ( 2 ) and ( 3 ) at 5 mM concentration. Assume [E T ] is the same in each experiment. [S] (mM) ( 1 ) ν (μmol mL -1 sec -1 ) ( 2 ) ν (μmol mL -1 sec -1 ) ( 3 ) ν (μmol mL -1 sec -1 ) 1 12 4.3 5.5 2 20 8 9 4 29 14 13 8 35 21 16 12 40 26 18 a. Determine V max and K M for the enzyme. b.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 07/23/2008 for the course BMB 401 taught by Professor Unknown during the Spring '04 term at Pennsylvania State University, University Park.

Page1 / 4

Problem_set_5 - Problem Set#5 BMB 401 Spring 2004 Problems from Lehninger 1 2 3 4 5 6 7 8 9 10 12 Problem 1 The kcat for alkaline

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online