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Unformatted text preview: biochemical studies. Its buffering range is pH 7 to 9, and K b is 1.19 x 10-6 for the aqueous reaction shown in the problem in the textbook. a. What is the optimal pH for TRIS buffers? b. Calculate the ratio of [TRIS]/[TRISH + ] at pH = 7.00 and at pH = 9.00. c. A buffer is prepared by diluting 50.0 g TRIS base and 65.0 g TRIS hydrochloride (written as TRISHCl) to a total volume of 2.0 L. What is the pH of this buffer? What is the pH after 0.50 mL of 12 M HCl is added to a 200.0 mL portion of the buffer?...
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- Summer '04
- pH, buffer solution, BUFFER QUESTION KEY