CHM447 2003 Test 1 - CHM 4-475 {IflflfiS} Bio-Organic...

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Unformatted text preview: CHM 4-475 {IflflfiS} Bio-Organic Chemistry In-elass test #1 EMS 1. .fitn enzyme inhibition experiment is found to give the series of Lineweaver-Eturlt plots shown below as the concentration of inhibitor is increased. Increaer lll'l lll=|2 tea] {a} Draw a kinetic scheme consistent with this observation. (5 marks) {h} Assuming that enzymeasubstratc and enzyme-inhibitor complexes are in lasts equilibrium with free enzyme, derive a rate equation for your scheme in Michaelis‘ lvlenten form. {15 marl-ts) {e} Show that this equation [converted to Lincweaver Burk form] will explain the observed data. {5 marks] 2. Chyrnotrypsi'n is a member of the serine protease family of enzymes. {i} 1Why are these enzymes called “serine” proteases? {5 marks} (ii) What is the substrate specificity of chymotrypsinr.’ {5 marks) {iii} Draw a chemical mechanism for die hydrolysis of a peptide bond by the enzyme ehymotrypsin. [1:3 marks] {iv} What is die role of the oxyanion hole? {l II] marks) (v) What is the role of the SI pocket? {lfl marks} 3. if Serl 95 of ehymotrypsin is mutated to alanine the enzyme is much less effective but still catalyses the hydrolysis of esters and amides. Presumably the role of the serine residue is assumed by a water molecule from the solvent. {i} Draw a kinetic scheme for the reaction catalyzed by the mutant enzyme {1 Cl marks} {ii} Assuming a steady state for the the concentration of any intermdieates derive a rate equation for your scheme in lvlichaelis—Menten form. {15 marks} {iii} What are ltcm and KM in this case? {i markst E51; 11M " by h = £51153 k’ a I : LEEJEEJ ALWK align-6%” [914' “Gfl—Pfikw FEMMJ JIM) LE”; Wk; Til-{.4 Tr?“ -‘ F‘- ciccw-U *Lua Lcficfl , J gimme ’9' F5“ filohabha LcmrI-M‘ fa E: '3‘ f:- f" I H H ___r :5; A! rd FF- : _______._ g; "— N m ~31 ~ 1 m“ fl Hf _ I 4:" -— f'w f N u “1 g j} M #N“ FL _ M _ fir f1: M jer‘ I 1P, {L} a w‘fiurw”: 51¢: '“ x, J r ) f. d‘r‘f'l'culfj fltff' Hl'i - a f. 4wq? fim m it“ c Wm gh—flja-fl H E “Ifan n»- . [:3 H“ m E ______ (if SUP ‘1 J \a HréF'O-f “if” \rjer Wily— ,IH can __L «a. t‘ __ OH H {.2 “JR? H WM "‘ I ELIE/'7?» u. H mknufie rfi‘uw ( gin-m I fa. A L? EFF-— dimfig duh-{q $1. H Tl FINE-inner 2 1'? gig? fili DH '5- H Hflfifilnr’ we“ I "Hill-H g f E— [4* r E g F f“ 'wr aw ‘— ° U ‘5 ( } N we 1 J Emir- wmcw—w , Wamw/ {L511 C’Lx‘tb'hx. M P1 5W ' ‘WF I 9-3“ a (1 F 4 LL } I; + E E. E8 “—== E 4 F” b4 :11: ECU‘ fl-fl%% ffi‘fifi‘f} (L) gm: Mia? rm 455;: O : L ijf:] h Lr! {Ex} —-L1_ [F57 O} {as-J " £1513 (“H ier (a) [E] : [E1] (er F21) Jan Ff : [ESE (Karin-)l 4- I) __ T 1?? . j‘a '| {:1 32,; gut : F 111% [5? L, 3%: I + f é—P‘Lfil ( 4. 252 f N” ...
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This note was uploaded on 07/23/2008 for the course CHM 447 taught by Professor Woolley during the Fall '00 term at University of Toronto- Toronto.

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CHM447 2003 Test 1 - CHM 4-475 {IflflfiS} Bio-Organic...

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