CHM447 2004 Test 1

CHM447 2004 Test 1 - CHM HTS{101353 Bia-Dtgnnie Chenfietty...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Background image of page 5
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: CHM HTS {101353) Bia-Dtgnnie Chenfietty Isl—class test #1 EDEN- 1. :‘m enzyme inhihilinu experiment 15 found It: give the entries of LhiE‘Jr‘tflVttI-flurk pic-ts shown Est-law as the canceutte ticm oFisthihitut is Increased. 1WX1U'3 rn-t-rrn-tTran-n-l-rrH-rn-t—ln-n-n—n-rrn-q- El};— 1. II'Itl'lt-arfimg-[I] "' EU 'I' __ | E 40 " v _. a If 2|} . . fwd-"— a: —Zfl -.-.-_uJ.-.I.I.-_|_u_l.+ uJ—LLuJ—u.u_u.I.-_Ll m . 0.4 I15 B B 1 D 1915] (a) [3er a kinetic scheme consistent with this observation. {5 marks} {is} Assuming that enzyme-substrate and enwme-Jnl'lihitu: templexes are in fest—equiiihlium with ftee enzyme. decier n sate equation for your scheme in MiehueLisuMemen Farm. [15 marks) it} Show Lhilt this eqtmion (converted to Lintwenvet Emit Fun-t1) will explain the uhfitu'lr'cd data. (5 maths) 2. Trypsirl is a metnlser trf the sen'ne pct-tease famiif of magmas. {i} Why :Lte these L'HmeCE cnhed “setine” pretest-.5? {5 merits} {ii} Wth is the stdsstnte swtleciticitfrr uftq'psiu? {5 maths} [itl'j- Dtaw :1 chemical mechanism For the hydmiysis nfn pepfide hand by the enzyme crypt-in. {13 marks} {it} ‘What :is the tale of the fixrertiun hole? fill] maths) [tr] Wth Is the tale of the 51 packet? {10 maths) 3. {ESL-.1195 tri'd'tymtsttypsin is mutated to alanine the enzyme is much less effective hut sljl'l catnIyzes the hydtuiysls Elf esters and amides. Presumzthiy rhe tale clfd1e seine tesitlue is assumed by a water molecule me the suivent. {3} Draw a kinetic scheme fer the reaction catalyzed by the mutant muyme {It} maths] {ii} Assuming n stentl};r state for the toucenttatinu uf my huennetliatts deti't'e :1 mt: equation t'nr you: scheme in hhchaelis-Meuten fut-tn. {I5 maths) Til-Tier are hmtenti KM 1n thls case? {5 maths} - ' mg; g - H.— Fr Tm §i____—_ . 2E " I fifialmimir H f ckualn‘i Clflmra'teg {9 Roi; Hawk m 51¢in In «it; MMM¢ _mrwgmfifi SHEA-um . :hvu. €*‘§5W¥1__ M12 I‘E‘F‘BJ‘m-xebit £5?) (1'14; 95m. .m fig" WW4? v) 5» flaws?“ {Wrui JuLfiM_ I g flflgjfltfl ELISE]: C.LLa-4:'_L_ a} a. 3&6 M 5...:ch ’ 0+ o’ij .hq Q1 {.4925 913:1) - (ff-Muir R sufii.t_ _WF _ 'IL'I 5L2. 1 — E (1*, .£:+ 5:? _E 5 “—? 5+? m Arjl aazjm Epf'cm-‘J Lil) run- h2.[?51. .0? _ flair c; ; L~__Ertr1£sj —L..[fs]*h1[efl _ (a) A _ .05} 1‘ 82.51 (3.3 $1 M £34 a) [E1 -= [ET [LL-1.41.1”- Efl he; _‘__ I JET 5 14. . __ (m #1) It ' I ...
View Full Document

This note was uploaded on 07/23/2008 for the course CHM 447 taught by Professor Woolley during the Fall '00 term at University of Toronto.

Page1 / 5

CHM447 2004 Test 1 - CHM HTS{101353 Bia-Dtgnnie Chenfietty...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online