lac operon notes - 13.4 Regulation of Gene Expression What...

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13.4 –Regulation of Gene Expression What is one of the ways that bacteria adapt to specific environmental conditions? By altering the levels of mRNA available for translation. From energetic stand point how would bacteria control translation? By controlling the initiation and transcription to prevent the unnecessary use of nucleotide triphosphates and consequent energy- costly synthesis of protein. Control of transcription initiation, responding to physical or chemical cues can be divided into two classes. At the Transcription level 1. Negative Control a. There are many ways to decrease the capacity of RNA polymerase to transcribe a specific gene or operon: i. A repressor protein bind to specific sites (operators), near or within promoter. 1. The binding of the repressor prevents transcription by blocking the polymerase from binding its operator, or if already bound, from moving forward. b. E.g. Lac operon and trp operon i.
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