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Unformatted text preview: J. Colton Riley Wednesday, July 16, 2008 Lab 2 Study questions: 1. The 3 major issues in using a light microscope are magnification, contrast, and resolution. Magnification is the limit of total magnification of an image by combination of objective and ocular lenses. Resolution is the ability of a microscope to produce an image in which two objects separated by a short distance are shown as two objects rather than one. Contrast is most components of biological specimens that transmit light equally. (Parts have low contrast.) 2. Biologists use phase contrast because contrast is produced in apparently transparent cells without killing the cells and also artifacts are not produced. 3. Abbes equation is = . d 0 612 nsin and it defines the resolving power of a microscope. NA is the numerical aperture which is the denominator of Abbes equation ( nsin ). NA and resolving power are inversely related, as one increases the other decrease and vice versa. NA is also inversely related with working distance. The depth of field of higher NA objectives must be smaller. 4. Bacteria were encountered during part J, Demonstrations of Living Specimens in the fourth section when we were observing a wet mount of a suspension of...
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- Spring '08