Exam2_SUMMER_2008_KEY

Exam2_SUMMER_2008_KEY - GENE3200 Summer 2008 Bedell Exam II...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
GENE3200 – Summer 2008 Bedell Exam II Name____________________________________StudentID______ KEY _______________ 1 Write your name and ID number (810 number, NOT SSN) on every page The exam should be completed in ink. Regrades will not be given if you write in pencil. Unless indicated otherwise, there is only one correct answer per question. All requests to re-grade an exam must be made in writing to either Dr. Bedell or Ms. Roche-Rios within 48 hours of the grades being posted on WebCT. 1. (2 points) Transduction in bacteria involves __________________________________ A). the adsorption of phage to both host and recipient bacteria. B). the adsorption of phage to neither host nor recipient bacteria. C). the adsorption of phage to host bacteria but not to recipient bacteria. D). the adsorption of phage to recipient bacteria but not to host bacteria. 2. (1 point) True or False . In specialized transduction, any gene or set of genes may be transferred from the donor bacterium to the recipient bacterium. 3. (1 point ) True or False. A temperate phage can remain within the host cell for a period of time without killing the host. 4. (2 points) A bacterial lysogen is ______. A) a cleared area in a bacterial lawn B) a type of bacteriophage that infects E. coli C) a bacterial strain with a prophage D) a bacterial strain that is resistant to bacteriophage infection E) a bacteriophage that cannot infect bacteria 5. (4 points) Let’s say you want to do a gene transfer experiment with a generalized transducing phage. After growing the phage on the appropriate donor bacterial strain, you wanted to be sure that you had obtained a good phage lysate. So, you tested for re-infection of the donor bacteria and found the expected number of plaques. Since your phage lysate was good, you then used it to infect a recipient bacterial strain and plated the latter cells on the correct selection media for your experiment. However, despite multiple attempts, you fail to obtain any transductants. Which of the following is/are the most likely explanation(s) for what went wrong: A) The donor bacterial strain was resistant to infection by that phage B) The recipient bacterial strain was resistant to infection by that phage C) The donor bacterial strain was defective for recombination D) The recipient bacterial strain was defective for recombination E) None of the above F) All of the above G) A or C H) B or D
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
GENE3200 – Summer 2008 Bedell Exam II Name____________________________________StudentID______ KEY _______________ 2 6. A map of a trp + met + arg + ala + ade + pro + bacterial strain is shown to the right. This strain is known to be lysogenic for a newly discovered phage, but the site of the prophage is not known. You use this lysogen as a source of transducing phages, which is then used to infect a trp _ met _ arg _ ala _ ade _ pro _ bacterial strain. After a short incubation, samples of the infected bacteria are plated on six different media, with the media
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 07/30/2008 for the course GENE 3200 taught by Professor Condie during the Summer '08 term at UGA.

Page1 / 10

Exam2_SUMMER_2008_KEY - GENE3200 Summer 2008 Bedell Exam II...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online