140-S08-Feb29 - Important piece of information for Monday:...

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Unformatted text preview: Important piece of information for Monday: Last names beginning A-L: 101 Barker Last names beginning M-Z: 240 Mulford Testing site: (1) Deletion mapping of genes (Benzer) using the segregational test for allelism: (2) Deletion mapping of genes (Bridges) using the functional test for allelism: If you can , the point mutant is outside the region deleted. If it is , the point mutant is outside the region deleted. Can you recover a wildtype allele from the mutant hybrid? (a recombination test ) Is the mutant hybrid phenotype wildtype? (a complementation test ) Deletion mapping based on a complementation test Fig. 14.8 w- rst- fa- Df(1)256-45) = w- (mutant) phenotype ( failure to complement) hence white is within region deleted . phenotype Bulge in the synapsed polytene chromosomes shows what is deleted. Inversions also have helped locate genes on the Drosophila polytene chromosome map via complementation tests Inversion breakpoints may knock out bw The complementation test as an operational definition of the gene is not quite as straightforward as it may sound. genes can have very complex complementation patterns because of all the various kinds of information they contain that work only in cis (and all the various ways in which that information can be changed by mutation ) p227 Heading: rII regon has two genes Is this statement compatible with the statement that complementation groups are what we want to call genes? (starting bottom of p291): A gene is not simply the DNA that is transcribed into the mRNA codons specifying the amino acids of a particular polypeptide. Rather, a gene is all the DNA sequences needed ( IN CIS ) for expression of the gene into a polypeptide product. A gene therefore includes the...
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140-S08-Feb29 - Important piece of information for Monday:...

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