140-S08-Mar10 - Terminology for chromosomal rearrangements:...

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Terminology for chromosomal rearrangements: (used in connection with Muller's mutant allele category tests to manipulate gene dose) Df = deficiency = deletion Bulge in the synapsed polytene chromosomes of a heterozygote shows what is deleted Fig. 14.8 A physical map of the genome in Muller's day (used to reduce allele dose)
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Terminology for chromosomal rearrangements: (used in connection with Muller's mutant allele category tests to manipulate gene dose) Dp = duplication (insertional vs. tandem) (used to increase allele dose) Df = deficiency = deletion (used to reduce allele dose) Bulge in the synapsed polytene chromosomes of a heterozygote shows what is deleted (or duplicated ) what is deleted Such rearrangements helped workers locate genes on the polytene chromosome map.
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Inversions also have helped locate genes on the Drosophila polytene chromosome map Inversion breakpoints ( In )
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fertilized egg (embryo) 1st instar larva 2nd instar larva 3rd instar larva Pupa ( metamorphosis ) 1 day 1 day 1 day 2 days 4 days adult ¢ (reproductive in ~2 days) XX XY If a gene functions at different times to do different things, temperature shifts of ts mutants can reveal those various functions Drosophila life cycle @25C
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determination of the “temperature-sensitive period” ( TSP ) by single temperature shifts up or down point (time) in development when temp. shifted embryo larva pupa adult ultimate phenotype all OK all mut restrictive unshifted permissive unshifted shift rstrctv to prmsv shift prmsv to restrv TSP start : when begins drop TSP end : when wildtype TSP single
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determination of the “temperature-sensitive period” ( TSP ) point (time) in development when exposed to rstrctv. temp for some fixed period of time (relatively short for high resolution). embryo
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This note was uploaded on 08/01/2008 for the course MCB 140 taught by Professor Urnov during the Spring '08 term at University of California, Berkeley.

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140-S08-Mar10 - Terminology for chromosomal rearrangements:...

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