1.beyond.WC.DNA

1.beyond.WC.DNA - MCB 110:Biochemistry of the Central Dogma...

Info iconThis preview shows pages 1–12. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
MCB 110:Biochemistry of the Central Dogma of MB Prof. Nogales Part 3. Membranes, protein secretion, trafficking and signaling Part  2. RNA & protein  synthesis. Prof. Zhou Part 1. DNA  replication,  repair and  genomics (Prof. Alber)
Background image of page 2
MCB 110:Biochemistry of the Central Dogma of MB Part  2. RNA & protein  synthesis. Prof. Zhou Prof. Nogales Part 3. Membranes, protein secretion, trafficking and signaling Part 1. DNA  replication,  repair and  genomics (Prof. Alber)
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
DNA structure summary 1 1. W & C (1953) modeled average DNA (independent of sequence) as an:  anti-parallel, right-handed, double helix   with   H-bonded base pairs on  the inside and the sugar-phosphate backbone on the outside . Each chain runs 5’ to 3’ (by convention). Profound implications: complementary strands suggested mechanisms of  replication, heredity and recognition. Missing Structural variation in DNA as a function of sequence Tools to manipulate and analyze DNA (basis for  biotechnology, sequencing, genome analysis)
Background image of page 4
DNA schematic (no chemistry) 3. Duplex strands are  antiparallel and  complementary.  Backbone outside; H-bonded bases  stacked inside. 1. DNA strands  are directional 1.  Nucleotide =  sugar-phosphate     + base 4. The strands  form a double  helix
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Nucleic-acid building blocks nucleoside nucleotide glycosidic bond
Background image of page 6
Geometry of DNA bases and base pairs! C G T A H-bonds satisfied Similar width Similar angle to glycosidic bonds Pseudo-symmetry of 180° rotation
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Major groove and minor groove definitions Major groove Minor groove Subtended by the glycosydic bonds Opposite the glycosydic bonds
Background image of page 8
Comparison of B DNA and A DNA (formed at different humidity) bp/turn Base tilt Major groove Minor groove P-P distance 10 small wide Narrow 6.9 Å 11 20° narrow & deep wide & shallow 5.9 Å (winds around) 3.4- 3.6 Å Bps near helix axis Bps off helix axis
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Average structure of dsRNA (like A DNA) “side”  view “End”  view  3’ 5’ Minor groove  shallow and wide    Major groove deep  and narrow  (distortions needed  for proteins to  contact bases)   Twist/bp  ~ 32.7° ~ 11 bp/turn Bases tilted
Background image of page 10
DNA structure varies with sequence 1. “Dickerson dodecamer” crystal structure 2. Twist, roll, propeller twist and displacement
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 12
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 08/01/2008 for the course MCB 110 taught by Professor Alber,zhou,nogales during the Fall '07 term at Berkeley.

Page1 / 51

1.beyond.WC.DNA - MCB 110:Biochemistry of the Central Dogma...

This preview shows document pages 1 - 12. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online