Jacob_Discuss

Jacob_Discuss - Microsecond Folding of the Cold Shock...

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Microsecond Folding of the Cold Shock Protein Measured by a Pressure-Jump Technique Discussion questions: may be asked on the in-class portion of the exam 1) Describe some of the goals of authors of the present paper 2) What are cold shock proteins? 3) How can one experimentally identify a protein as a cold shock protein? 4) Why is the kinetics of cold shock proteins relatively easy to study? 5) What contributions to the field of protein folding were made by the early (1970’s) stopped-flow studies? 6) Why is photochemical triggering of a folding reaction suitable for the study of folding of redox enzymes such as cytochrome c, but not others, such as HIV protease? 7) What happens if a solution of unfolded protein is compressed such that the pressure increases significantly? 8) What happens is a solution of folded protein is compressed such that the pressure increases significantly? 9) What happens is a solution of unfolded protein is expanded such that the pressure decreases significantly?
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Jacob_Discuss - Microsecond Folding of the Cold Shock...

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