Lecture_2_ESM214_05

Lecture_2_ESM214_05 - Microbes, Enzymes, Kinetics Lecture 2...

Info iconThis preview shows pages 1–18. Sign up to view the full content.

View Full Document Right Arrow Icon
Microbes, Enzymes, Kinetics Lecture 2 ESM 214 W’05
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Prokaryote Eukaryote Cellular makeup
Background image of page 2
What cells need and do cell cell reproduction, anabolism C arbon nutrients e nergy electron acceptor metabolic byproducts environmental conditions: pH, moisture, temperature, salinity
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Terminology (“trophs”) in microbial metabolism Energy source Chemo= chem bonds Organo = organic Litho= inorganic Photo= sunlight e- acceptor Aerobic = oxygen Anaerobic= not O 2 Carbon Hetero- = organic Auto- = inorganic
Background image of page 4
Nomenclature based on energy sources
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Examples Anaerobic chemo-organo heterotroph e.g. denitrifying Pseudomonas • NO 3 - as an e - acceptor breaks chemical bonds for energy Organic C as energy and C source Aerobic chemolithoautotroph e.g. ammonia oxidizing bacteria Nitrosomonas • O 2 as terminal electron acceptor Break chemical bonds for energy • Energy comes from breaking down NH 4 + • CO 2 as C cource
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Concept: energy uses and orgins How is the energy used? 1) transport 2) biosynthesis (anabolism) 3) polymerization 4) maintenance (regulation of pH, osmotic pressure, motility) All biochemical reactions = metabolism; anabolic are biosynthetic; catabolic are energy generating Where does energy come from? Breaking chemical bonds (inorganic or organic substrates) = chemotrophs Sunlight= phototrophs
Background image of page 10
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 12
Enzymes: key concepts proteins that catalyze biological reactions have quarternary structure can chemically / physically denature and renature specific to specific reactions; except those involved in co- metabolic reactions typically act unidirectionally re-available for catalysis after reaction naming indicates type of reaction and substrate intracellular or extracellular constituitive or induced
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 14
= fermentation = respiration
Background image of page 15

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 16
Recap Microbial metabolism described by C & e - sources and by terminal electron acceptor All are required for microbial processes Metabolism = anabolism + catabolism Enzymes catalyze oxidation-reduction reactions in metabolism Enzymes can be specific (to rxns, microbes) Respiration: external e - acceptor – Aerobic = O 2 ; anaerobic = other; facultative = either Fermentation: substrate e - acceptor
Background image of page 17

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 18
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 48

Lecture_2_ESM214_05 - Microbes, Enzymes, Kinetics Lecture 2...

This preview shows document pages 1 - 18. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online