11 - Cys2Cys10 and Cys6Cys103 intact pH 5.0, 0.25 M NaCl...

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Factors Affecting the Stability of RNase T1 Coulombic Interactions  pH 7.0, 25 °C Gln25 Lys (creates salt bridge), pH 7.0, 25 °C Glu58 Ala (destroys salt bridge), pH 7.0, 25 °C pH  pH 2, 25 °C (net charge = 6) pH 4.5, 25 °C (net charge = 0) pH 7.0, 25 °C (net charge = –8) pH 10, 25 °C (net charge = –12) Ligand  pH 7.0, 25 °C pH 7.0, 25 °C + 0.2 M Na 2 HPO 4 G unfolding (kcal/mol)  5.6 6.5 4.8 4.5 8.9 5.6 3.0 5.6 10.0 TIBS 1990 , 15 , 14
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Factors Affecting the Stability of RNase T1 Disulfide Bonds 
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Unformatted text preview: Cys2Cys10 and Cys6Cys103 intact pH 5.0, 0.25 M NaCl Cys2CH 2 CO 2 , Cys10CH 2 CO 2 , Cys6Cys103 intact pH 5.0, 0.25 M NaCl Cys2CH 2 CO 2 , Cys10CH 2 CO 2 , Cys6CH 2 CO 2 , Cys103CH 2 CO 2 pH 5.0, 0.25 M NaCl Temperature pH 7.0, 25 C pH 7.0, 6 C pH 7.0, 48 C G unfolding (kcal/mol) 10.2 6.8 0.9 5.6 8.3 TIBS 1990 , 15 , 14 Protein Stability: The Small Difference between Large Numbers Data from Peter Privalov...
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This note was uploaded on 08/08/2008 for the course BIOCHEM 704 taught by Professor Kiessling during the Spring '08 term at Wisconsin.

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11 - Cys2Cys10 and Cys6Cys103 intact pH 5.0, 0.25 M NaCl...

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