Ulrike presentation

Ulrike presentation - Parallel Chemical Genetic and...

Info iconThis preview shows pages 1–11. Sign up to view the full content.

View Full Document Right Arrow Icon
Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets Eggert et al. 2004
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Cytokinesis
Background image of page 2
Cytokinesis (aurora B)
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
(Glotzer 2005)
Background image of page 4
RNAi and Chemical inhibitors RNAi library knockdown of specific genes Chemical libraries Cytokinesis inhibitors Cytochalasin: inhibits actin Blebbistatin: inhibits Myosin II
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Design of parallel screens Cultured Drosophila Kc 167 cells Added dsRNA or small molecules in 384-well plates Grew for 4 days in dsRNA or 2 days with small molecules Fixed, stained for cytoplasm and DNA or tubulin Automated fluorescence microscopy
Background image of page 6
Potential small molecule inhibitors Out of 50,000 possible molecules, 50 inhibited cytokinesis. Tested cross-reactivity with HeLa cells, BSC-1 cells, and drug resistant S. cerevisiae About 50% inhibited cytokinesis 7 inhibit all three Is this expected?
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Genome wide RNAi screen Out of 19470 dsRNAs, dsRNAs corresponding to 214 genes affected cytokinesis 20% previously implicated 1 new gene essential for cytokinesis
Background image of page 8
Figure 1 Small molecule inhibitors (25) RNAi (214)
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Figure 1 Small molecule inhibitors (25) RNAi (214) -Compare percentages of phenotype strength?
Background image of page 10
Image of page 11
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 26

Ulrike presentation - Parallel Chemical Genetic and...

This preview shows document pages 1 - 11. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online