mudpit - Sperm Chromatin Proteomics identifies conserved...

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Sperm Chromatin Proteomics identifies conserved fertility factors by D.S. Chu, H. Liu, P. Nix, T.F. Wu, E.J. Ralston, J.R. Yates III, B.J. Meyer
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Outline Background C. elegans breeding biology Basic mass spec. proteomics Paper Methods and Results Conclusions
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C. elegans, where men are men and women are, well… www.wormbook.org , www.wormclassroom .org
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Unique chromatin remodeling during development of male germ cells Nature 434: 583.
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Basic Mass Spec Proteomics Requires effective protein prep Effective Ionization Required for loading on Mass Spec Genome required for comparison to Mass Spectrum Aebersold and Mann (2003) Nature
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Variations of Mass Spec Tech Aebersold and Mann (2003) Nature
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MudPIT Technology Multidimensional Protein Identification Technology Two Dimensional LC Strong Cation Exchange Reverse Phase Tandem Mass Spec Rough abundance estimate from spectral sampling Washburn et al (2001) Nature Biotech Why do you want to use a 2D-LC?
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The Goal: Identify and characterize spermatogenic chromatin-associated proteins that are important for fertility Purification of chromatin from meiotic cells MudPIT analysis of factors co-purifying with chromatin Prioritization of sperm proteins based on
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mudpit - Sperm Chromatin Proteomics identifies conserved...

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