2DGelAnalysis - Establishment of a near-standard...

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Establishment of a near-standard two-dimensional human urine proteomic map J. Oh, J-H Pyo, R-H. Jo, S-II Hwang, S-C. Kang, J-H. Jung, E-K. Park, S-Y. Kim, J-Y. Choi, J. Lim Proteomics 2004, 4:3485-3497 Erica
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2-dimensional gel electrophoresis Powerful tool for characterizing complex protein mixtures. Up to 10,000 protein spots can be analyzed simultaneously 2-step process that separates proteins according to isoelectric focusing (IEF) and SDS- polyacrylamide gel electrophoresis.
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2-dimensional gel electrophoresis Powerful tool for characterizing complex protein mixtures. Up to 10,000 protein spots can be analyzed simultaneously 2-step process that separates proteins according to isoelectric focusing (IEF) and SDS-polyacrylamide gel electrophoresis. According to what protein property does each technique separate the proteins from the initial mixture? - IEF - SDS-PAGE
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Extraction of protein Isoelectric focusing (1D gel) Ettan™ IPGphor™ staining Spot excision; Trypsin digestion SDS-PAGE MALDI-TOF
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Mass Spec Principles Ionizer Sample + _ Mass Analyzer Detector
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This note was uploaded on 08/08/2008 for the course GEN 875 taught by Professor Glasner during the Fall '07 term at University of Wisconsin.

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2DGelAnalysis - Establishment of a near-standard...

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