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Numbers in Microbiology

Numbers in Microbiology - 2 nd ed 1996 pg 50-54 In E coli...

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Numbers in Microbiology NOTE: see references provided for original citations for each of these numbers. I. (Moat and Foster “Microbial Physiology”, 3 rd ed., 1995 pg 11.) E. coli reproduces once every 40 minutes Dry weight of an average cell is 2.5 x 10 -13 g Total weight of an average cell is 9.5 x 10 -13 g Of dry weight: Protein = 55% rRNA = 16.7% tRNA = 3% mRNA = 0.9% DNA = 3.1 % Lipid = 9.1 % Lipopolysaccharide = 3.4% Peptidoglycan = 2.5% Vitamins = 2.9% Inorganic ions = 1.0% Total cell volume = 9 x 10 -13 mL Periplasmic space = 30% of cell volume II. (Paul and Clark “ Soil Microbiology and Biochemistry”,
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Unformatted text preview: 2 nd ed., 1996, pg 50-54) In E. coli: • 20% of dry weight = RNA • 3% of dry weight = DNA • 81% of RNA in prokaryotes is in ribosomes with equal molecular amounts of 23S, 16S, and 5S rRNA • mean bacterial volume = 0.196 μ m 3 • bacterial specific gravity = 1.07 • dry matter in cell = 30% • C in dry cell matter = 44% • DNA bacterial content per cell = 4 x 10-15 g DNA per cell in lab puppies; 2 x 10-15 g per cell in soil organisms III. (Microbiology for Environmental Engineers, Gaudy & Gaudy, 1980. McGraw Hill, p. 59) Empirical formula for cells: C 5 H 7 NO 2...
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