Gerritse et al_AEM_1999v65p5212

Gerritse et al_AEM_1999v65p5212 - APPLIED AND ENVIRONMENTAL...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
A PPLIED AND ENVIRONMENTAL MICROBIOLOGY, 0099-2240/99/$04.00 1 0 Dec. 1999, p. 5212–5221 Vol. 65, No. 12 Copyright © 1999, American Society for Microbiology. All Rights Reserved. Influence of Different Electron Donors and Acceptors on Dehalorespiration of Tetrachloroethene by Desulfitobacterium frappieri TCE1 JAN GERRITSE, 1 OLIVER DRZYZGA, 2 * GEERT KLOETSTRA, 2 MISCHA KEIJMEL, 2 LUIT P. WIERSUM, 2 ROGER HUTSON, 3 MATTHEW D. COLLINS, 3 AND JAN C. GOTTSCHAL 2 TNO Institute of Environmental Sciences, Energy Research and Process Innovation, Department of Environmental Biotechnology, 7300 AH Apeldoorn, 1 and Department of Microbiology, University of Groningen, 9751 NN Haren, 2 The Netherlands, and Department of Food Science and Technology, University of Reading, Reading RG6 6AP, United Kingdom 3 Received 21 May 1999/Accepted 8 September 1999 Strain TCE1, a strictly anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene (PCE) and trichloroethene (TCE), was isolated by selective enrichment from a PCE-dechlorinating chemostat mixed culture. Strain TCE1 is a gram-positive, motile, curved rod-shaped organism that is 2 to 4 by 0.6 to 0.8 m m and has approximately six lateral flagella. The pH and temperature optima for growth are 7.2 and 35°C, respectively. On the basis of a comparative 16S rRNA sequence analysis, this bacterium was identified as a new strain of Desulfitobacterium frappieri , because it exhibited 99.7% relatedness to the D. frappieri type strain, strain PCP-1. Growth with H 2 , formate, L -lactate, butyrate, crotonate, or ethanol as the electron donor depends on the availability of an external electron acceptor. Pyruvate and serine can also be used fermentatively. Electron donors (except formate and H 2 ) are oxidized to acetate and CO 2 . When L -lactate is the growth substrate, strain TCE1 can use the following electron acceptors: PCE and TCE (to produce cis -1,2-dichlo- roethene), sulfite and thiosulfate (to produce sulfide), nitrate (to produce nitrite), and fumarate (to produce succinate). Strain TCE1 is not able to reductively dechlorinate 3-chloro-4-hydroxyphenylacetate. The growth yields of the newly isolated bacterium when PCE is the electron acceptor are similar to those obtained for other dehalorespiring anaerobes (e.g., Desulfitobacterium sp. strain PCE1 and Desulfitobacterium hafniense ) and the maximum specific reductive dechlorination rates are 4 to 16 times higher (up to 1.4 m mol of chloride released z min 2 1 z mg of protein 2 1 ). Dechlorination of PCE and TCE is an inducible process. In PCE-limited chemostat cultures of strain TCE1, dechlorination is strongly inhibited by sulfite but not by other alternative electron acceptors, such as fumarate or nitrate. Anaerobic microorganisms play a key role in the dehaloge- nation (4, 8, 11, 29, 43) and eventual mineralization (9, 11, 14, 40) of many chlorinated and fluorinated contaminants. Studies of the physiology and biochemistry of these organisms have revealed that they may transform a wide range of chlorinated aliphatic and aromatic compounds cometabolically (11, 29, 41)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 08/06/2008 for the course ESM 219 taught by Professor Holden during the Fall '07 term at UCSB.

Page1 / 10

Gerritse et al_AEM_1999v65p5212 - APPLIED AND ENVIRONMENTAL...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online