Experiment 2: Osmosis - Direction and Concentration Gradients In this experiment, we will investigate the effect of solute concentration on osmosis. A semi- permeable membrane (dialysis tubing) and sucrose will create an osmotic environment similar to that of a cell. This selective permeability allows us to examine the net movement of water across the membrane. You will begin the experiment with a 30% sucrose solution, and perform a set of dilutions to create lower concentration solutions. Materials (3) 100 mL Beakers (3) 250 mL Beakers (1) 10 mL Graduated Cylinder (1) 100 mL Graduated Cylinder Permanent Marker **8 Rubber Bands (2 blue, 2 green, 2 red, and 2 yellow) 60 g Sucrose (Sugar) Powder, C 12 H 22 O 11 *Paper Towels *Scissors *Sheet of Saran Wrap or Aluminum Foil *Stopwatch *Water ***(4) 15 cm. Pieces of Dialysis Tubing **Contains latex. Please handle wearing safety gloves if you have a latex allergy. *You Must Provide ***Be sure to measure and cut only the length you need for this experiment. Procedure 1. Use the permanent marker to label the three 250 mL beakers as 1, 2, and 3. 2. Cut four strips of dialysis tubing, each 15.0 cm long. Fill Beaker 3 with 100 mL of water and submerge the four pieces of dialysis tubing in the water for at least 10 minutes. 3. While the dialysis tubing soaks, reconstitute the sucrose powder according to the instructions provided on the bottle´s label (your kit contains 60 g of sucrose in a chemical bottle). This will create 200 mL of a 30% stock sucrose solution.
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- Spring '14
- Sucrose, Semipermeable membrane, rubber bands, sucrose solution