TOPIC 14 - TOPIC 14: REPLICATION pp. 556 - 572. Read the...

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TOPIC 14: REPLICATION pp. 556 - 572. Read the material on prokaryotes as this will help you understand the whole topic, but concentrate on eukaryotes. For now, do not worry about DNA damage and repair. We may or may not cover this, only time will tell. DNA synthesis is called replication and is semi-conservative. DNA synthesis is directional, nucleotides can only be added at the 3' end of each of the two polynucleotide strands which make up the complete DNA molecule. DNA synthesis begins at replication origins and occurs at replication forks. pp. 556 - 572 (Lots in here but you will need to study this in order to fully understand what we have covered. Remember, the text is not to be used to expand the information we have covered, but rather to help you better understand what we have covered. Please note: Things are a bit different in the pro- and eukaryotes. In spite of the fact that the text slightly emphasizes the system in prokaryotes, we are going to emphasize replication, transcription and translation as they occur in eukaryotes . There are a few things which you should know about prokaryotes and I will point them out below or in lecture. But to fully understand the system in the eukaryotes you will have to study the material on the prokaryotes. Also, the text gives considerable detail so you will have to use common sense as to how much of this you need to know. The lectures will certainly provide a fair estimate of this. No I will not be more specific! REPLICATION . Replication is the synthesis of DNA. Because of the specific base pairing (complimentary nature of DNA), upon separation of the DNA double strand, each strand reproduces a complimentary strand. As each newly synthesized double helix contains one strand from the original molecule and one new strand, it is referred to as semi-conservative replication or synthesis (i.e., half is conserved). When a deoxynucleoside triphosphate
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This note was uploaded on 08/06/2008 for the course BIO 1140 taught by Professor Fenwick during the Winter '07 term at University of Ottawa.

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TOPIC 14 - TOPIC 14: REPLICATION pp. 556 - 572. Read the...

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