ex2sg_2008 - Study Guide for Exam 2 Zoology 470 2008 Exam...

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Study Guide for Exam 2 – Zoology 470 – 2008 Exam format Remember that you can expect a good chunk of the exam to be short answer questions (i.e., 1-2 sentence response). Other types of questions, including identification, multiple choice, matching, and true/false, will also be present, but don't count on them being the largest portion of the exam. In addition, there has been an emphasis in class on experiments and what they tell us about mechanisms of early development. These are really important, and you should understand them thoroughly. The text should be used to reinforce in-class material. Your best study hints will come from last year’s exam. Remember that it’s on the course web site. Important reminder: You are responsible for knowing the cleavage patterns presented in Gilbert, Ch. 8, and representative examples of each. Topics for Exam 2 Egg activation What is egg activation ? What ionic events are important for full egg activation (e.g., calcium , pH increase )? How does intracellular pH change following fertilization? What experiments indicate the pH rise is required for the onset of protein synthesis? What experiments demonstrate that protein synthesis is essential immediately after fertilization for normal development, and that mRNA production is eventually required? Pronuclear migration What cytoskeletal system and molecular motor drive pronuclear migration, and what role is the sperm aster thought to play in this process? Cleavage and the Blastula 1. Cleavage : What cytoskeletal systems and molecular motor protein is involved? How does the cell cycle during early cleavages differ from that of cells in the adult (somatic cells)? What are cyclins ? What are reductive cleavages ? What does this mean for the embryo? What are the basic types of cleavage , and what organisms are good examples of each type? What is the difference between radial and spiral cleavage , and what groups of organisms display these types of cleavage? How does the amount and position of yolk affect how cleavage occurs? What significant accomplishments take place during cleavage (e.g., regionalization, blastocoel formation , etc.)? How are astral microtubules thought to stimulate where the cleavage furrow forms? Can you cite an experiment that shows this? What is Rho , and why is it important in stimulating cleavage furrows? 2. Blastula structure : How does the yolk content of the egg affect blastula structure? The Anatomy of Genes What are the basic steps in the production and processing of mRNA? How does chromatin structure affect the possibility of transcription? What are the functions of the various regions of DNA associated with the production of a functional mRNA (e.g., enhancers/silencers or regulatory elements, promoter region)? What are the functions of various parts of an mRNA (e.g., introns , exons , 3'-untranslated region , poly-A "tail" )?
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Zoo 470 2007 – Study Guide for Exam 3 Page 2 Transcriptional regulation of specific genes - 1. DNA-binding proteins and DNA regulatory domains
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ex2sg_2008 - Study Guide for Exam 2 Zoology 470 2008 Exam...

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