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Unformatted text preview: Zoology 470 - Quiz #1 2008
This quiz has 10 questions and is worth a total of 10 points. It is designed to test basic knowledge about molecular techniques used in developmental biology. Use the accompanying answer sheet to enter your answers for the following multiple choice questions. There is one right answer for each question. Please make sure you enter your student ID number on the answer sheet. 1. Which of the following techniques provide information about the relative abundance of protein in a tissue sample? a. Southern blotting b. Immunoblotting c. In situ hybridization d. a & c e. b & c 2. You are studying the expression of the APC protein in a mouse embryo. Which of the following techniques could result in an increase in expression of APC protein? a. RNA mediated interference (RNAi) against the APC RNA b. Production of a APC "knockout" mouse by homologous recombination c. Production of an otherwise normal mouse containing a wild-type APC transgene d. a & b e. All of the above 3. You prepare a tissue sample from a mouse embryo and place it under a microscope Which technique would you use to identify in which cells of an early mouse embryo APC mRNA is normally expressed? a. Indirect immunostaining b. Northern blotting c. In situ hybridization d. Southern blotting e. none of the above 4. You were in the Yamanaka lab when they discovered that embryonic stem cells express 42 genes at higher levels than other cultured cells. Which technique did you probably use to discover this? a. Southern blotting b. DNA sequencing c. "Knockouts" d. DNA microarrays e. none of the above 5. You suspect that a mouse mutant carries a mutation in the mRNA that encodes the APC protein, but your work is complicated because you must work with single cells. Which of the following is likely a step you would NOT use to determine if your suspicions are correct? a. Direct mRNA sequencing b. PCR c. DNA sequencing d. Production of cDNA e. All are appropriate Zoo 470 Quiz 1 2007 - Page 2 6. Studying genomic DNA is useful because a. It reflects the kinds and abundance of mRNAs present in a tissue sample b. It contains the regulatory DNA that affects expression of a gene c. It lacks introns, which complicate studying genes d. All of the above are reasons genomic DNA is useful e. None of the above is a reason genomic DNA is useful 7. Antibodies are useful for studying proteins except for which of the following reasons? a. They are useful for detecting a specific protein b. They allow detection of the size of a protein on an immunoblot (Western blot) c. They can be used to detect the location of a protein in a tissue sample d. They can sometimes be used to disrupt the function of a protein e. All of these are valid reasons for using antibodies 8. A zygotic mutation is best characterized by which of the following? Assume the mutation is recessive. a. It results in phenotypes in the offspring only when the offspring carry two mutant copies of the gene b. It results in phenotypes in the offspring when the mother is mutant regardless of the genotype of the father c. It results in phenotypes in the offspring when the father is mutant regardless of the genotype of the mother d. It results in phenotypes only when the offspring have one mutant and one normal copy of the gene e. All of the above statements are incorrect 9. The polymerase chain reaction (PCR) is useful because a. It can amplify DNA from extremely small amounts of starting material b. It can be used to amplify specific DNA sequences c. It is useful as a plot element in TV crime dramas such as C.S.I. d. a, b & c e. a & b [Note: we accepted e. as a correct response.] 10. RNAi mediated interference is useful because a. It usually results in reduction in the level of DNA containing a particular gene b. It usually results in reduction of the level of mRNA transcribed from a particular gene c. It usually results in reduction in the level of protein encoded by a particular gene d. a, b, & c e. b & c ...
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This note was uploaded on 08/08/2008 for the course ZOO 470 taught by Professor Hardin during the Spring '08 term at Wisconsin.
- Spring '08