ex1_2007 - Name: _____________________________ Student...

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Unformatted text preview: Name: _____________________________ Student ID:_________________ Page 1 Zoo 470 - Exam 1 - Spring 2007 February 27, 2007 This exam has a total of 6 pages and a total of 50 points . You will have 90 minutes to complete it. Please put your name and student ID on all pages. Good luck! 1. Provide brief definitions for each of the following ( 6 points ): a. capacitation: b. Smad: c. parthenote: d. compaction: e. fate mapping: f.: broadcast spawning: 2. MPF and cytostatic factor are known to regulate meiotic cell divisions. Answer the following questions, based on your knowledge of these factors' effects on cell division. a. If purified CSF were injected into one cell of a two-cell embryo immediately after first cleavage, what would you predict would happen at the time of the next division? Clearly state your reasoning. ( 1 point ) Name: _____________________________ Student ID:_________________ Page 2 2 (cont) b. Recall that frog eggs can be artificially activated by pricking them with a needle. What would you expect would happen to levels of MPF within a few minutes after pricking an egg? Clearly state your reasoning . ( 2 points ) 3. You have isolated a gene that you believe encodes a novel sperm cell surface protein, headline , and you are making a presentation to a major pharmaceutical corporation for research support to work on this protein. a. You come to the presentation armed with the following pieces of data. For each of the following facts, state the technique(s) you used to obtain your data. ( 4 points ) Discovery Technique(s) Used for Discovery When viewed under the microscope, human sperm extracted from the epidydimis lack headline protein The mouse headline mRNA has a size of 2.6 kB Knocking down headline protein in male zebrafish without reducing mRNA levels results in sperm that cannot fertilize eggs You isolate a single primary spermatocyte from the testis of an infertile man, and you determine that the headline mRNA produced by this cell has a mutation at nucleotide 267. b. headline mutant sperm are inefficient at undergoing the acrosome reaction. Suppose you find that headline encodes a calcium channel protein that allows calcium to enter sperm cells when they contact the zona pellucida. Describe one experiment that you could perform that would show that if the normal ionic events that trigger the acrosome reaction occur, the mutant sperm will undergo a normal acrosome reaction. ( 2 points ) c. Headline has multiple roles in development. You learn that C. elegans headline is a zygotic gene required for embryonic survival. If a mother heterozygous for a recessive mutation in headline mates with a father heterozygous for the same mutation, what percentage of her offspring would you expect to survive? (1 point) Percentage of dead offspring: Name: _____________________________ Student ID:_________________ Page 3 4. Regulation of reproduction in humans involves a number of secreted factors. 4....
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This note was uploaded on 08/08/2008 for the course ZOO 470 taught by Professor Hardin during the Spring '08 term at Wisconsin.

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ex1_2007 - Name: _____________________________ Student...

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