Differentiated es cells what technique did they use

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Unformatted text preview: human ES cells vs. differentiated ES cells. What technique did they use to do this? (1 point) Technique: DNA microarray ("gene chip") 4. Recall that mouse ES cells require leukemia inhibitory factor (LIF), produced by "feeder cells" to maintain pluripotency in culture. G-Q Zhao's lab at the University of Texas Southwestern Medical School showed earlier this year that one type of mouse feeder cells(called "C-STO" cells) were not as good at maintaining ES pluripotency than others, and set about trying to identify why (Hao et al., 2006). Hao et al (2006) identified 27 genes encoding secreted factors whose expression was reduced in C-STO cells. They then made transgenic cells expressing some of these genes, and examined their ability to support ES cells. a. Two of the genes they tested, when expressed in cultured cells, resulted in significantly lower levels of -catenin phosphorylation among ES cells when the cells were co-cultured. What type of secreted protein did these two genes likely enc...
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