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Unformatted text preview: otency. [partial credit for epiblast, because the problem asks about after organogenesis] d. Where within the cells that produce Nanog protein would you expect it to be found? (1 point) Location within cells: Nucleus (it's a transcription factor) 2. You set out to examine the relationship between Nanog and Oct-4. Design an experiment that would test whether Nanog expression requires Oct-4 function. Clearly state your experimental procedures, and your expectations if Nanog expression depends on Oct-4 function. Assume you can perform any experiment involving mouse cells discussed in class, and that you can detect gene expression using any technique discussed in class. (5 points) Experiment: Examine Nanog expression in an oct-4 homozygous knockout mouse, using in situ hybridization or immunostaining. This assumes the oct-4 KO exist. If not, we would have to generate it using homologous recombinant as discussed in class. [Would also accept RNAi instead of KO; would also accept work done in ES cells instead of in the embryo] [Some may answer that occt-4 is required for an ICM, in which case they may say that there is nothing to test in the "outcome se...
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This note was uploaded on 08/08/2008 for the course ZOO 470 taught by Professor Hardin during the Spring '08 term at Wisconsin.

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