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ps3_2008_key - Zoo 470 2008 Problem Set#3 Name Student...

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Zoo 470 2008 Problem Set #3 Page 2 Name:________________________________ Student Number:__________________ If you worked in a group, other collaborators: _____________________________________________ 1. Nanog encodes a homeodomain-containing protein, and is thought to be necessary for maintenance of pluripotency only after cells in the mouse embryo become committed to either trophoblast or embryonic fates, and is not expressed until this time. a. What technique could you use to detect where Nanog mRNA is expressed in the early embryo? ( 1 point ) Technique : in situ hybridization b. In what cells of the "morula" stage embryo (i.e., the 16-32 cell embryo) would you expect Nanog mRNA to be expressed in? State your reasoning . ( 3 points ) Interior cells, which are pluripotent, and give rise to the ICM of the blastocyst c. What cells in the later mouse embryo (i.e. after organogenesis is underway) would you expect to express Nanog mRNA? State your reasoning . ( 2 points ) Primordial germ cells, which retain pluripotency. [partial credit for epiblast, because the problem asks about after organogenesis] d. Where within the cells that produce Nanog protein would you expect it to be found? ( 1 point ) Location within cells : Nucleus (it’s a transcription factor) 2. You set out to examine the relationship between Nanog and Oct-4. Design an experiment that
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