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Paper_Clip_PCR_0001 - Name: Paper CIip PCR In this activity...

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PaperCIip PCRName:Inthisactivityyouwillbeperlorming PCR(polymerasechain reaction) usingpaperclipstorepresentDNA.Eachcoloredpaper cliprepresentsaparticular nucleotide intheDNAcode:Blue-Adenine(A)R.ed-Thymine(T)'=-.';.-::-Guanine(G)-Cytosine(C)White-hydrogen bondstoconnectDNAstrands1.Startbycreatingyour templateorstartingDNAstrandoutof paperclips.Connect thepropercolorsof paper clipstorepresent thefollowingDNAsequence:A-G-A-T-C-G-C-A-A-A-G-C-A-T-TKeepellstrandsflato* yalnr desk!2.Usingyourfirststrandas aguide,qeatethecomplementary (matching)strandpropercolorsofpaper clips.Hint:ApairswithTandGpairswithC. UsetwowhiteioCoror"ttheendsofyour twoDNAstrands together.Yourtemplatestrandshouldbe3. Createyour Primersoutofthefollowingsequences:out ofthepaper clipson top.Primer1:T-A-G.-=-;'.--=Primer2:G-C-A..=---..l4. Repeatstepthreetomakea secondpairof primers.Nowyouarercady toperform PCR"o*rau*r',!ilH!O"Bg:SPaperCIiPPCRv.l'3ovER....
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/Human-Heredity-Principles-and-Issues-11th-Edition-9781305251052-1185/
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Chapter 13 / Exercise 12
Human Heredity: Principles and Issues
Cummings
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Blue(A)Reci(T)i,,..:'.::'r(G)(C)5.Beginbydenatrrirgorseparatingyour twoDNAstrands (removewhitepape-rclips).6.Attach(oranneal)Primer1tothetemplate (top)strandinitsproperplaceusingawhitepape-rclip.

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Term
Spring
Professor
f
Tags
DNA, Bottom Strand
We have textbook solutions for you!
The document you are viewing contains questions related to this textbook.
Human Heredity: Principles and Issues
The document you are viewing contains questions related to this textbook.
Chapter 13 / Exercise 12
Human Heredity: Principles and Issues
Cummings
Expert Verified

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